Biodegradation – OECD 301F manometric Respirometry Test Method
The respirometry test system is a 20-channel electrolytic respirometer manufactured by Co-ordinated Environmental Services, Ltd. (Cornwall UK). The system is based on a proven oxygen generating process coupled to a sensitive manometric cell.
- 20-channel C.E.S. respirometer is ideal for testing poorly soluble, volatile and adsorbing compounds
- Accurately measures ultimate and ready biodegradability
- GLP Verifiable results: the system has built in data storage facilities and also produces an hourly hard copy print-out of the raw data
- Specialty testing: lubricant base stocks, greases, hydraulic and gear oils, PAOs, additive packages, drug intermediates and waste streams
- Optional acclimated vs. un-acclimated microbial inocula [ASTM D-5864]
- Short-term 14-Day Biodegradation Screening for R&D purposes
- Local, domestic WWTP consistency
- Preliminary results can be e-mailed at any point during conduct of the biodegradation study
- Final report securely e-mailed on Day 29
- Discounted testing for multiple test substances
Scope: The test substance is placed in a sample flask and sealed by a manometric cell/CO2 trap and immersed in a temperature stabilized water bath and stirred by a magnetically coupled stirrer. As the biodegradation process starts, the microorganisms consume O2 converting it to CO2 during aerobic respiration. The CO2 produced is absorbed by a solution of 10N NaOH in the CO2 trap causing a net reduction in gas pressure within the sample flask. This pressure reduction is detected by the manometric cell and triggers the electrolytic process. The electrolytic process generates O2, which restores the pressure in the sample flask. The magnitude and duration of the electrolyzing current is proportional to the amount of oxygen supplied to the microorganisms.